To fully understand equine color genetics, one must first understand a few basic genetic principles. I will go over them first and hopefully give you a solid basis on which to build your genetic knowledge.
A gene is the smallest segment of DNA capable of producing a specific trait. A trait is defined as a distinct variant in the phenotypic character of an organism. A simple example of a trait would be the white color of a flower. White in flowers is a genetically inherited characteristic, aka, a trait. A genotype is the genetic makeup of any given organism, whereas a phenotype is the outward appearance of the organism. We’ll discuss how they relate to each other later on. For now, we are going to take a close look at genotypes.
Each gene is made up of two alleles, one inherited from each parent. It is in this fashion that organisms reproduce in their likeness (a white flower produces another white flower). Each allele is passed at a 50% rate, ie allele1 (let’s say white) is passed 50% of the time and allele2 (let’s say red) is passed 50% of the time. When an organism has two identical alleles (two reds or two whites) it is said to be homozygous for that gene. If it carries one of each allele (one red and one white) it is considered heterozygous. More on what that means later.
So let’s look at this from a more mathematical perspective. This is where the famous “punnet square” comes in. A punnet square is a diagram used to predict the outcome of a particular cross. Dominant alleles are generally denoted by a capital letter representing the gene the allele is “on” (the red gene for this example). In the case of red flowers vs. white flowers (using “simple dominance”, which we are for now), red would be ‘R’ and white would be ‘r’. This is a punnet square predicting the possible outcome of two flowers who are both heterozygous (thus carrying one each of the red and white alleles):
This square shows us that there are four possible genotypes for this particular gene mating. 25% will be homozygous for the red allele, 25% will be heterozygous by receiving a red allele from Mom and a white allele from Dad, 25% will be heterozygous by receiving a white allele from Mom and a red allele from Dad, and 25% will be homozygous for the white allele.
As you can see, often times each parent passes a different form (allele) of any given gene (ie one passes the white allele and the other passes the red allele) causing a bit of a conflict. Such disputes are solved by a set order of dominance. There are three different types of dominance. In equine color genetics, you will need to know a bit about each type of dominance so I will give you some examples to work with.
Simple dominance: In flowers (for this example), red is dominant over white so the flower inheriting both a red and a white allele will be red. That is a classic example of simple dominance. That flower will be able to produce either red or white flowers depending on the genetics of its mate and is thus considered to be heterozygous for that gene. If the flower were grown with two red alleles instead it would still be red but it would be considered homozygous for the red allele. Due to the red allele’s dominance, a flower carrying even one red allele will always be red. Any flower produced from a homozygous red parent will always be red regardless of the other parent’s contribution as the homozygous parent only has red to pass. A flower homozygous for white can only pass the white allele so the color of any flowers created from it will ultimately be determined by the flower it is crossed with. Two flowers homozygous for the white allele can only create other white flowers. The same goes for homozygous reds of course. Let’s look at a couple more punnet squares so you can see how different zygosity of the parents can affect the resulting flower.
Now you can see how one parent being homozygous for a given allele will affect matters:
With Mom being homozygous for the dominant allele (red), you can see all flowers will inherit a dominant allele from her. In this case all of her flowers will be red with 50% of them inheriting Dad’s recessive allele and having the ability to produce white flowers themselves.
In this case Mom’s being homozygous for the recessive allele means that each of her flowers will have the ability to produce white flowers but due to Dad’s dominant allele, only 50% of them will be white themselves.
Incomplete dominance: Another form of dominance you will need to understand is the “incomplete dominant”. In the case of an incomplete dominant the dominant allele has a different expression in the homozygous state. Whereas our flower which is heterozygous for red/white is red and our flower which is homozygous for red is also red, an incomplete dominant gene’s expression would mean the flower would have a distinctly different appearance when comparing the heterozygote to the homozygote. In this example the heterozygous flower would be *pink*, an even blend of the two alleles, and the homozygous red flower would be red. The homozygous white flower would still be white. The inheritance of an incomplete dominant gene is the same, only the phenotypical expression varies.
When writing a punnet square for an incomplete dominant trait, it is better to use capital letters for both alleles, separating them by phenotype instead. We’ll use R and W.
So here you can see that 25% will be red, 50% will be pink and 25% will be white.
Co dominance: In addition to simple dominance and incomplete dominance there is one more type of dominance that will impact your understanding of equine color genetics. Co-dominance in simple terms means that both alleles are dominant and they work together. Using our red and white flowers example, an RR flower would be red, WW would be white and RW would be *striped*, a combination of the two alleles but not a blend like the incomplete dominant. Red and white share equal turf in this instance instead of melding together. The inheritance of a co-dominant gene is also the same, only the expression differs.
So here you can see that 25% will be red, 50% will be striped and 25% will be white.
Now that you probably have a good understanding of the basics of genetic inheritance, we’ll move on to the specific equine color genes and their impact on the equine phenotype.